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    Carl Zeiss scanning electron microscopy zeiss crossbeam 340
    The induced membrane possesses an architecture that resembles the native periosteum. (A) HE staining of the native periosteum and induced membrane. Scale bar, 200 μm. The boxed area is enlarged at the right. The outer fiber layer is representative at the top and the inner cambium layer is at the bottom. Scale bar, 100 μm. (B) Thickness of the native periosteum and induced membrane. (C) TEM images of the native periosteum. Scale bar, 500 nm. (D) The number of fibers and (E) the distance between adjacent fibers in the inner and outer zone. Immunohistochemical staining of OCN and BMP-2 in the inner and outer zone in the native periosteum (F) and induced membrane (G). Scale bar, 100 μm. Semiquantitative analysis of OCN and BMP-2 expression in the native periosteum (H) and induced membrane (I) based on immunohistochemical staining. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. IZ, inner zone; OZ, outer zone; TEM, transmission electron <t>microscopy;</t> OCN, osteocalcin; BMP-2, bone morphogenetic protein 2.
    Scanning Electron Microscopy Zeiss Crossbeam 340, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Reverse biogradient biomimetic periosteum with osteogenic and angiogenic characteristics for bone regeneration"

    Article Title: Reverse biogradient biomimetic periosteum with osteogenic and angiogenic characteristics for bone regeneration

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.101967

    The induced membrane possesses an architecture that resembles the native periosteum. (A) HE staining of the native periosteum and induced membrane. Scale bar, 200 μm. The boxed area is enlarged at the right. The outer fiber layer is representative at the top and the inner cambium layer is at the bottom. Scale bar, 100 μm. (B) Thickness of the native periosteum and induced membrane. (C) TEM images of the native periosteum. Scale bar, 500 nm. (D) The number of fibers and (E) the distance between adjacent fibers in the inner and outer zone. Immunohistochemical staining of OCN and BMP-2 in the inner and outer zone in the native periosteum (F) and induced membrane (G). Scale bar, 100 μm. Semiquantitative analysis of OCN and BMP-2 expression in the native periosteum (H) and induced membrane (I) based on immunohistochemical staining. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. IZ, inner zone; OZ, outer zone; TEM, transmission electron microscopy; OCN, osteocalcin; BMP-2, bone morphogenetic protein 2.
    Figure Legend Snippet: The induced membrane possesses an architecture that resembles the native periosteum. (A) HE staining of the native periosteum and induced membrane. Scale bar, 200 μm. The boxed area is enlarged at the right. The outer fiber layer is representative at the top and the inner cambium layer is at the bottom. Scale bar, 100 μm. (B) Thickness of the native periosteum and induced membrane. (C) TEM images of the native periosteum. Scale bar, 500 nm. (D) The number of fibers and (E) the distance between adjacent fibers in the inner and outer zone. Immunohistochemical staining of OCN and BMP-2 in the inner and outer zone in the native periosteum (F) and induced membrane (G). Scale bar, 100 μm. Semiquantitative analysis of OCN and BMP-2 expression in the native periosteum (H) and induced membrane (I) based on immunohistochemical staining. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. IZ, inner zone; OZ, outer zone; TEM, transmission electron microscopy; OCN, osteocalcin; BMP-2, bone morphogenetic protein 2.

    Techniques Used: Membrane, Staining, Immunohistochemical staining, Expressing, Transmission Assay, Electron Microscopy

    Fabrication and characterization of the reverse biogradient biomimetic periosteum. (A) TEM images of Fe 3 O 4 , nHA, nHA-AL and Func-HA. Fe 3 O 4 and Func-HA; scale bar, 100 nm. nHA and nHA-AL, scale bar, 200 nm. (B) SEM images showing the surface morphology of the biomimetic periosteum in the inner and outer zone. Scale bar, 20 μm. (C) Pore size distributions of the inner and outer zones. (D) TEM images of Func-HA in the nanofibrous scaffolds. Scale bars, 100 μm and 100 nm. (E) Release profiles of BMP-2 and AL into PBS from the biomimetic periosteum. TEM, transmission electron microscopy; SEM, scanning electron microscopy; nHA, nanocrystal hydroxyapatite; nHA-AL, nanocrystal hydroxyapatite-alendronate; Func-HA, functionalized hydroxyapatite; BMP-2, bone morphogenetic protein 2; AL, alendronate.
    Figure Legend Snippet: Fabrication and characterization of the reverse biogradient biomimetic periosteum. (A) TEM images of Fe 3 O 4 , nHA, nHA-AL and Func-HA. Fe 3 O 4 and Func-HA; scale bar, 100 nm. nHA and nHA-AL, scale bar, 200 nm. (B) SEM images showing the surface morphology of the biomimetic periosteum in the inner and outer zone. Scale bar, 20 μm. (C) Pore size distributions of the inner and outer zones. (D) TEM images of Func-HA in the nanofibrous scaffolds. Scale bars, 100 μm and 100 nm. (E) Release profiles of BMP-2 and AL into PBS from the biomimetic periosteum. TEM, transmission electron microscopy; SEM, scanning electron microscopy; nHA, nanocrystal hydroxyapatite; nHA-AL, nanocrystal hydroxyapatite-alendronate; Func-HA, functionalized hydroxyapatite; BMP-2, bone morphogenetic protein 2; AL, alendronate.

    Techniques Used: Pore Size, Transmission Assay, Electron Microscopy



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    The induced membrane possesses an architecture that resembles the native periosteum. (A) HE staining of the native periosteum and induced membrane. Scale bar, 200 μm. The boxed area is enlarged at the right. The outer fiber layer is representative at the top and the inner cambium layer is at the bottom. Scale bar, 100 μm. (B) Thickness of the native periosteum and induced membrane. (C) TEM images of the native periosteum. Scale bar, 500 nm. (D) The number of fibers and (E) the distance between adjacent fibers in the inner and outer zone. Immunohistochemical staining of OCN and BMP-2 in the inner and outer zone in the native periosteum (F) and induced membrane (G). Scale bar, 100 μm. Semiquantitative analysis of OCN and BMP-2 expression in the native periosteum (H) and induced membrane (I) based on immunohistochemical staining. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. IZ, inner zone; OZ, outer zone; TEM, transmission electron <t>microscopy;</t> OCN, osteocalcin; BMP-2, bone morphogenetic protein 2.
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    Image Search Results


    The induced membrane possesses an architecture that resembles the native periosteum. (A) HE staining of the native periosteum and induced membrane. Scale bar, 200 μm. The boxed area is enlarged at the right. The outer fiber layer is representative at the top and the inner cambium layer is at the bottom. Scale bar, 100 μm. (B) Thickness of the native periosteum and induced membrane. (C) TEM images of the native periosteum. Scale bar, 500 nm. (D) The number of fibers and (E) the distance between adjacent fibers in the inner and outer zone. Immunohistochemical staining of OCN and BMP-2 in the inner and outer zone in the native periosteum (F) and induced membrane (G). Scale bar, 100 μm. Semiquantitative analysis of OCN and BMP-2 expression in the native periosteum (H) and induced membrane (I) based on immunohistochemical staining. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. IZ, inner zone; OZ, outer zone; TEM, transmission electron microscopy; OCN, osteocalcin; BMP-2, bone morphogenetic protein 2.

    Journal: Materials Today Bio

    Article Title: Reverse biogradient biomimetic periosteum with osteogenic and angiogenic characteristics for bone regeneration

    doi: 10.1016/j.mtbio.2025.101967

    Figure Lengend Snippet: The induced membrane possesses an architecture that resembles the native periosteum. (A) HE staining of the native periosteum and induced membrane. Scale bar, 200 μm. The boxed area is enlarged at the right. The outer fiber layer is representative at the top and the inner cambium layer is at the bottom. Scale bar, 100 μm. (B) Thickness of the native periosteum and induced membrane. (C) TEM images of the native periosteum. Scale bar, 500 nm. (D) The number of fibers and (E) the distance between adjacent fibers in the inner and outer zone. Immunohistochemical staining of OCN and BMP-2 in the inner and outer zone in the native periosteum (F) and induced membrane (G). Scale bar, 100 μm. Semiquantitative analysis of OCN and BMP-2 expression in the native periosteum (H) and induced membrane (I) based on immunohistochemical staining. Data are presented as mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. IZ, inner zone; OZ, outer zone; TEM, transmission electron microscopy; OCN, osteocalcin; BMP-2, bone morphogenetic protein 2.

    Article Snippet: The morphology of the biomimetic periosteum was analyzed using scanning electron microscopy (SEM, Zeiss, Crossbeam 340) at 2 kV.

    Techniques: Membrane, Staining, Immunohistochemical staining, Expressing, Transmission Assay, Electron Microscopy

    Fabrication and characterization of the reverse biogradient biomimetic periosteum. (A) TEM images of Fe 3 O 4 , nHA, nHA-AL and Func-HA. Fe 3 O 4 and Func-HA; scale bar, 100 nm. nHA and nHA-AL, scale bar, 200 nm. (B) SEM images showing the surface morphology of the biomimetic periosteum in the inner and outer zone. Scale bar, 20 μm. (C) Pore size distributions of the inner and outer zones. (D) TEM images of Func-HA in the nanofibrous scaffolds. Scale bars, 100 μm and 100 nm. (E) Release profiles of BMP-2 and AL into PBS from the biomimetic periosteum. TEM, transmission electron microscopy; SEM, scanning electron microscopy; nHA, nanocrystal hydroxyapatite; nHA-AL, nanocrystal hydroxyapatite-alendronate; Func-HA, functionalized hydroxyapatite; BMP-2, bone morphogenetic protein 2; AL, alendronate.

    Journal: Materials Today Bio

    Article Title: Reverse biogradient biomimetic periosteum with osteogenic and angiogenic characteristics for bone regeneration

    doi: 10.1016/j.mtbio.2025.101967

    Figure Lengend Snippet: Fabrication and characterization of the reverse biogradient biomimetic periosteum. (A) TEM images of Fe 3 O 4 , nHA, nHA-AL and Func-HA. Fe 3 O 4 and Func-HA; scale bar, 100 nm. nHA and nHA-AL, scale bar, 200 nm. (B) SEM images showing the surface morphology of the biomimetic periosteum in the inner and outer zone. Scale bar, 20 μm. (C) Pore size distributions of the inner and outer zones. (D) TEM images of Func-HA in the nanofibrous scaffolds. Scale bars, 100 μm and 100 nm. (E) Release profiles of BMP-2 and AL into PBS from the biomimetic periosteum. TEM, transmission electron microscopy; SEM, scanning electron microscopy; nHA, nanocrystal hydroxyapatite; nHA-AL, nanocrystal hydroxyapatite-alendronate; Func-HA, functionalized hydroxyapatite; BMP-2, bone morphogenetic protein 2; AL, alendronate.

    Article Snippet: The morphology of the biomimetic periosteum was analyzed using scanning electron microscopy (SEM, Zeiss, Crossbeam 340) at 2 kV.

    Techniques: Pore Size, Transmission Assay, Electron Microscopy